Figure 1.

Hiwi siRNA decreases the levels of mRNA and protein in cervical cancer cells. (A) The level of Hiwi mRNA in cervical cancer cells was analyzed using reverse transcription-quantitative polymerase chain reaction. The relative level of Hiwi mRNA was normalized to GAPDH. (B) The level of Hiwi protein in cervical cancer cells was detected using western blotting with β-actin as the internal reference. (C) The relative level of Hiwi protein in each group was calculated. All experiments were repeated three times, and the results are presented as the mean ± standard deviation. **P<0.01 vs. control cells. ***P<0.001 vs. control cells. siRNA, small interfering RNA.