Figure 3.

Downregulation of Hiwi arrests the cell cycle in cervical cancer cells. (A) The cell cycle of cervical cancer cells was detected using flow cytometry. (B) The percentage of cells in the G1 phase was calculated. (C) The level of cyclin A protein was detected using western blotting. (D) The relative level of cyclin A protein in each group was calculated. (E) The protein level of cyclin D1 was detected using western blotting. (F) The relative level of cyclin D1 protein in each group was calculated. All experiments were repeated three times, and the results are presented as the mean ± standard deviation. *P<0.05, **P<0.01 vs. control cells. siRNA, small interfering RNA.