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. 2018 Jun 28;16(3):2789–2796. doi: 10.3892/ol.2018.9036

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Copyright: © Sun et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Construction, production and infection of rAAV-Endostatin. (A) Construction of rAAV-endostatin vector. The endostatin gene was inserted into the MCS of pAAV vector; OS-RC-2 cells infected with rAAV-YRFP. (B) The rAAV particles in tumor cells are indicated by transmission electron microscopy (magnification, ×80,000). (C) Endostatin production in OS-RC-2 cells infected with rAAV-Endostatin was analyzed by ELISA. Comparison between RCC-rAAV-RYFP cells and OS-RC-2-rAAV-endostatin cells was performed using analysis of variance. *P<0.05. MCS, multiple cloning site; ITR, inverted terminal repeats; AAV, adenovirus-associated vector; EYFP, enhanced yellow florescent protein; ori, origin of replication.