Figure 1.

Autophagy inhibitor CQ induces apoptosis of QBC939 cells. (A) QBC939 cells were treated with various doses of CQ for 24 h and cell viability was determined by an MTT assay. Data are presented as mean ± standard deviation, n=3. (B) Cells were treated with 50 µM CQ for 12 and 24 h and then stained with Hoechst 33258. Cell morphology was observed by fluorescence microscopy (scale bar, 50 µm). The apoptosis ratio of QBC939 cells was detected by flow cytometric analysis after treatment with 50 µM CQ for 12 and 24 h. CQ, chloroquine.