Figure 2.

Autophagy inhibitor CQ initiates the intracellular death-inducing signaling complex-associated apoptosis pathway. (A) Expression of apoptosis-associated proteins and caspase-8 was analyzed by western blotting in QBC939 cells treated with CQ (50 µM). (B) Quantification of LC3, Bak, Bax, c-caspase3 and c-PARP. (C) Quantification of c-caspase8 and c-caspase8. *P<0.05 vs. control. C, cleaved; LC3, microtubules associated protein light chain 3; Bax, B-cell lymphoma 2-associated X; Bak, B-cell lymphoma 2 antagonist/killer; PARP, poly(ADP-ribose) polymerase; CQ, chloroquine.