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Combined effect of Notch1 siRNA and doxorubicin on gastric cancer cell apoptosis. (A) The apoptosis was measured by flow cytometer. (B) Notch1, NICD and apoptosis-associated proteins were determined by western blotting. (C) Analysis of relative caspase-3 activity in SGC7901. *P<0.05, **P<0.01, ***P<0.001 vs. control siRNA or as indicated. NICD, Notch1 intracellular domain; p53, tumor protein p53; Bcl-2, B-cell lymphoma-2; siRNA, small interfering RNA; Dox, doxorubicin.
