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. 2018 Jun 22;16(3):3169–3176. doi: 10.3892/ol.2018.9012

Figure 5.

Figure 5.

miR-376a regulated cell apoptosis by targeting FOXP2 in lymphoma. (A) The gene sequences of FOXP2 regulated by miR-376a. (B) The relative luciferase activities in wild-type 3′-UTR of FOXP2 and mutant FOXP2 3′-UTR in transfected cells. (C) The relative mRNA and protein levels of FOXP2 in transfected cells detected by RT-qPCR and western blot analysis. (D) The relative mRNA and protein expression of FOXP2 following cell transfection with si-FOXP2 detected by RT-qPCR and western blot analysis. (E) Percentage of apoptotic cells. (F) Representative images of the apoptosis assay following cell transfection with miR-376a overexpression vectors and si-FOXP2. Data were expressed as mean ± standard deviation (SD). *P<0.05 and **P<0.01 vs. control. miR-376a, microRNA-376a; RT-qPCR, reverse transcription quantitative polymerase chain reaction; FOXP2, forkhead box protein P2; 3′-UTR, 3′-untranslated region; si-FOXP2, small interfering RNA targeting FOXP2; FITC, fluorescein isothiocyanate; PI, propidium iodide; WT, wild type; Mut, mutant; NS, not significant.