Variations in hepatic functional reserve after irreversible electroporation in the 29 patients of the present study. Bonferroni's multiple comparison was used for comparison of values between the stated time points. The number of results varied as follows: 22, 18, 18, 16, 10, 15, 8, 9, 6, 8 and 4: 22 results were obtained on days 0, 1–2, 3–4, 5–6, 7–8, 9–10 and 11–20, respectively. Days on which <3 test results were obtained were merged with adjacent days. The markers of hepatic functional reserve were (A) alanine transaminase, (B) aspartate transaminase, (C) direct bilirubin and (D) total bilirubin. ***P<0.001 vs. IRE, irreversible electroporation; ALT, alanine transaminase; ALT, aspartate transaminase; D.BIL, direct bilirubin; T.BIL, total bilirubin.