Variations in hepatic functional reserve in 22 patients without hepatitis B. Bonferroni's multiple comparison was used to compare values across time points. The number of results varied between days: 48, 16, 12, 10, 6, 11, 10 and 12: 18 results were obtained on days 0, 1, 2, 3, 4, 5, 6–7, 8–10 and 11–20, respectively. Days on which <3 test results were obtained were merged with adjacent days. The hepatic functional reserve markers were (A) alanine transaminase, (B) aspartate transaminase, (C) direct bilirubin and (D) total bilirubin. **P<0.01 and ***P<0.001 vs. Day 0, 1 or 3. IRE, irreversible electroporation; ALT, aspartate transaminase; D.BIL, direct bilirubin; T.BIL, total bilirubin.