Variations in hepatic functional reserve after irreversible electroporation in 15 patients with hepatocellular carcinoma. Bonferroni's multiple comparison was used to compare values between different time points. The number of results obtained varied across time points as follows: 20, 16 16, 16, 15, 8, 9, 6 and 4: 22 results were obtained on Days 0, 1–2, 3–4, 5–6, 7–8, 9–10 and 11–20, respectively. Days on which <3 test results were obtained were merged with adjacent days. The hepatic functional reserve markers used were (A) alanine transaminase, (B) aspartate transaminase, (C) direct bilirubin and (D) total bilirubin. ***P<0.001 and **P<0.01 vs. Day 0, 1 or 3 IRE, irreversible electroporation; ALT, aspartate transaminase; D.BIL, direct bilirubin; T.BIL, total bilirubin.