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. 2018 Jun 25;16(3):2823–2832. doi: 10.3892/ol.2018.9016

Figure 3.

Figure 3.

TPX2 expression and function in prostate tumor cell lines. (A) Western blotting detected TPX2 protein expression in PCa cell lines. TPX2 was highly expressed in the DU145 cell line, while expressed at low levels in the PC3 and LNCaP cell lines. The CCK8 assay indicated that the overexpression of TPX2 in (B) LNCaP and (C) PC3 cells promoted the proliferation rate of PCa cells compared with the control group. The flow cytometry assay conducted in (D) LNCaP-NC and (E) LNCaP-TPX2 cells suggested that TPX2 inhibited cell cycle transit from G0/G1 to S/M phase in LNCaP-TPX2 cells. (F) The average proportion of cells in S+G2 phase was 40.607±2.172 in the LNCaP-TPX2 group, while it was 47.09±1.804 in the LNCaP-NC group (P=0.016). (G) PC3-NC and (H) PC3-TPX2 cells were also examined using flow cytometry. (I) No significant difference in PC3 cells was observed (P>0.05). TPX2, Targeting protein for Xenopus kinesin-like protein 2; PCa, prostate cancer; NC, negative control; NS, not significant. *P<0.05.