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. 2018 Jul 5;16(3):3489–3498. doi: 10.3892/ol.2018.9081

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Knockdown of survivin and/or XIAP in HGC-27 cells. (A) Reverse transcription-quantitative polymerase chain reaction analysis of survivin and XIAP mRNA expression in HGC-27 cells transfected with control non-sense, survivin, XIAP or survivin/XIAP siRNA. (B) Western blot analysis of XIAP, survivin and GAPDH protein expression levels. Lane 1, untreated HGC-27 cells; lane 2, control nonsense siRNA-treated HGC-27 cells; lane 3, HGC-27 cells transfected with XIAP siRNA; lane 4, HGC-27 cells transfected with survivin siRNA; and lane 5, HGC-27 cells transfected with survivin/XIAP siRNA (n=3). Data are expressed as the mean ± standard error of the mean. *P<0.05 vs. control group (one-way analysis of variance). XIAP, X-linked inhibitor of apoptosis protein; si, small interfering RNA; NC, negative control (transfected with control non-sense siRNA).