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. 2018 Jun 29;16(3):3126–3134. doi: 10.3892/ol.2018.9051

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Copyright: © Shi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — TBMS1 downregulates the expression of miR-126-5p-targeted VEGF-A (A) The 3′UTR sequence of VEGF-A and its binding site on miR-126-5p. (B) RT-PCR detection of VEGF-A mRNA expression in TBMS1-treated NCI-H1299 cells. Data in each group are expressed as mean ± SD from three independent experiments. β-actin was used as an internal control. Compared with control, ***P<0.001. (C) Detection on the expression of VEGF-A by immunofluorescence staining under ×200 magnification (scale bar: 100 µm). Representative photomicrographs from repeated experiments are shown. VEGF-A was visualized with Cy3-labeled goat anti-rabbit IgG as bright white. TBMS1, Tubeimoside-1.