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. 2018 Jun 29;16(3):3126–3134. doi: 10.3892/ol.2018.9051

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Copyright: © Shi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — TBMS1 inactivates VEGFR2 mediated ERK pathway. (A) RT-PCR analysis of VEGFR-2, MEK1 and ERK mRNA expression levels. β-actin was used as an internal control. (B) Western blot analysis on VEGFR-2 protein expression and MEK1 and ERK1/2 phosphorylation levels. GADPH was served as an internal control. Representative blots are presented with corresponding densitometric analysis is shown. Data are given as mean ± SD from three independent experiments. Compared with control, **P<0.01, and ***P<0.001; compared with 5-FU, ^#P<0.05, ^##P<0.01. TBMS1, Tubeimoside-1.