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. 2018 Jul 12;16(3):3826–3832. doi: 10.3892/ol.2018.9142

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Copyright: © Huang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Bufalin-induced apoptosis in bladder cancer cells through inactivation of Na⁺-K⁺-ATPase. (A) The protein expression of Na+/K+-ATPase α1 and α3 was detected by western blot analysis. GAPDH was used as a loading control. (B) The mRNA level of Na⁺-K⁺-ATPase α1 α2 and α3 was detected by qRT-PCR with specific primers of α1 α2 and α3 isoforms. GAPDH was used as a loading control. Data are presented as means ± SD of three independent experiments (*P<0.05 vs. control group). qRT-PCR, quantitative real-time PCR.