Figure 3.

α3-Na+-K+-ATPase overexpression attenuated Bufalin-induced apoptosis in bladder cancer cells. (A) T24 cells were transfected with vectors containing sequences of α3 subunit of ATPase. Transfection efficiency was determined using western blot. (B) T24 cells overexpressing α3 isoform of ATPase were treated with Bufalin for 2 h. The protein levels of α3 isoform, Bcl-2 and cleaved caspase-3 were detected using western blot. GAPDH was used as a loading control. (C) Annexin V-FITC/PI assay was performed to examine cell apoptosis in transfected T24 cells treated with Bufalin. Data are presented as the mean ± SD of three independent experiments (*P<0.05 vs. NC group or Bufalin + NC group). FITC, fluorescein isothiocyanate; PI, propidium iodide.