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. 2018 Jul 11;16(3):3587–3592. doi: 10.3892/ol.2018.9121

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Copyright: © Yin et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — miR-329 enhanced the sensitivity of CRC cells to 5-FU by decreasing the expression of E2F1. (A) The binding site in E2F1 mRNA for miR-329. (B and C) Luciferase reporter assay confirmed the direct binding of E2F1 and miR-329 in HCT116 and SW480 cells. (D) E2F1 expression was significantly higher in drug-resistant cells than that in non-resistant cells. (E) The E2F1 expression was significantly reduced after upregulation of miR-329. (F and G) Overexpression of E2F1 significantly increased cell viability in HCT116 and SW480 cells, while overexpression of both E2F1 and miR-329 partially reversed the cell activity. CRC, colorectal cancer; 5-FU, 5-fluorouracil. *P<0.05; **P<0.01; ***P<0.001.