Table I.
RT-qPCR primer sequences.
| Gene | Sequence | Product size (bp) | TM (°C) |
|---|---|---|---|
| CD105-mouse | |||
| Forward | 5′-CCCTCTGCCCATTACCCTG-3′ | 11 | 59.5 |
| Reverse | 5′-GTAAACGTCACCTCACCCCTT-3′ | 7 | 57.6 |
| CD105-human | |||
| Forward | 5′-CGCCAACCACAACATGCAG-3′ | 15 | 57.3 |
| Reverse | 5′-GCTCCACGAAGGATGCCAC-3′ | 8 | 59.5 |
| GAPDH-mouse | |||
| Forward | 5′-AAGGATGAAGGAAGTGATTTG-3′ | 40 | 53.77 |
| Reverse | 5′-AAGAGGAACATCGTGGTAAAG-3′ | 3 | 55.62 |
| GAPDH-human | |||
| Forward | 5′-TGATGACATCAAGAAGGTGGTGAAG-3′ | 24 | 61.03 |
| Reverse | 5′-TCCTTGGAGGCCATGTGGGCCAT-3′ | 0 | 68.32 |
Bp, base pair; Tm, temperature; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; GADPH, glyceraldehyde-3-phosphate dehydrogenase.