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. 2018 Aug 10;11:261. doi: 10.3389/fnmol.2018.00261

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Copyright © 2018 van den Hurk, Erwin, Yeo, Gage and Bardy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Natural variation in expression of housekeeping genes in single neurons. TaqMan quantitative real-time PCR was performed on SMARTer cDNA generated from n = 56 single human neurons successfully isolated for Patch-seq to profile the expression levels of ACTB and GAPDH housekeeping genes. ACTB and GAPDH were readily expressed though at variable levels from cell to cell as revealed by both qRT-PCR (A) and RNA-sequencing (B). (C) Single-neuron expression of ACTB and GAPDH housekeeping genes is highly correlated between qRT-PCR and RNA-sequencing measurements.