Table 1.

Comparison of Patch-seq methods for multimodal profiling of single neurons.

	Patch-seq Bardy et al. (2016)	Patch-seq Cadwell et al. (2016, 2017)	Patch-seq Fuzik et al. (2016)
Patch-seq-analyzed cell type(s)	In vitro ESC/iPSC-derived human midbrain-like neurons	Ex vivo and in vivo mouse neocortex interneurons	Ex vivo mouse neocortex pyramidal cells and interneurons
Precautions against RNase	✓	✓	?
Transcriptome sampling method	Entire neuron isolation including dendrites and axon	Aspiration of cell soma contents	Aspiration of cell soma contents
Analysis of transcripts from distal neurites	✓	χ	χ
Addition of RNase inhibitor to internal solution (to reduce RNase activity)	χ	✓	χ
Addition of EGTA to internal solution (to chelate divalent cations that are cofactors for RNase)	✓	✓	✓
Single-cell RNA reverse-transcribed and amplified immediately (<4 h) after collection	✓	?	χ
scRNA-seq protocol	Smart-seq (SMARTer)	Smart-seq2	STRT-C1