Figure 2.

Expression of CDKN2B was localized to epithelial cells and decreased in regions of increased α-SMA. (A) Normal, nonfibrotic lung and IPF lung were immunostained for CDKN2B (red), E-cadherin (green), and DAPI (blue). Representative images (×100 magnification) from three different patient lines are shown. (B) IPF lung sections were immunostained for CDKN2B (red), α-SMA (green), and DAPI. Shown are representative images from two sections (top, ×100 magnification; bottom, ×200 magnification). Mean fluorescence intensity of CDKN2B was compared between regions of high α-SMA–expressing and low α-SMA–expressing cells (n = 3 different patients, one to six images taken from each patient). ****P < 0.0001, Student’s t test.