Figure 3.

BAL albumin and total protein levels in uninjured mice. BAL was done and cell-free lavage fluid was used to determine albumin and total protein levels by colorimetric assays. (A–C) This was performed in uninjured C57BL/6J male mice after euthanasia with ketamine/xylazine (injection), isoflurane, or CO₂, and using three forms of BAL: (A) instillation and withdrawal of 800 μL of isotonic saline performed three times, (B) passive fill and drainage to 20 cm H₂O performed once, and (C) passive fill and drainage to 20 cm H₂O performed three times. Data are from n = 5 mice per group per experiment, each of which was repeated twice. *P < 0.05, **P < 0.005, ***P < 0.0005, and ****P < 0.00005 between the different forms of euthanasia.