O3 BAL albumin and total protein based on the method of lavage or euthanasia. Male C57BL/6J mice were exposed to FA or O3 exposure at 2 ppm for 3 hours. At 24 hours after exposure, the mice were killed by isoflurane, CO2, or ketamine/xylazine injection, and underwent BAL (800 μL fill, one fill to 20 cm H2O, and three fills to 20 cm H2O). (A–C) The BAL fluid was assessed for albumin and total protein based on the method of euthanasia or lavage. Data from n = 5 mice per group per lavage group per method of euthanasia, each of which was repeated once. *P < 0.05 between the FA and O3 groups.