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. 2018 Jul 16;9(16):2807–2816. doi: 10.7150/jca.25680

Figure 1.

Figure 1

MT1G silencing is closely linked to DNA methylation. A) MT1G mRNA expression and B) methylation levels in HCC samples compared with the expression in their adjacent non-tumor tissues were analyzed in two genomic screening datasets (mRNA expression dataset: TCGA_LIHC_exp_HiSeqV2-2015-02-24 obtained from Cancer Browser; methylation levels dataset: lihc_tcga.tar obtained from CBioPortal; student's unpaired t-test, n = 50). C) The correlation between MT1G mRNA expression and methylation status was analyzed in HCC samples of the above two datasets (r = -0.4442, n = 371, p-value < 0.0001). D) The methylation status and mRNA expression levels in 1 normal immortalized liver cell line LO2 and 6 HCC cell lines (HepG2, Hep3B, Bel7402, SMMC7721, MHCC97L and MHCC97H) were measured by MSP (M: methylation, U: unmethylation) and semi-quantitative RT-PCR. E) The methylation status was further confirmed by bisulfite genomic sequencing. F) MT1G mRNA expression was partially restored after treatment with the demethylation reagent 5-Aza-dC in 4 HCC cell lines.