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. 2018 Jul 30;8(15):4181–4198. doi: 10.7150/thno.25707

Figure 3.

Figure 3

Trpm4 is up-regulated after SCI, and FFA blocks Trpm4 expression in SCI. (A) Double immunofluorescence labeling of capillaries for Trpm4 (green) and CD31 (red) in sections through the penumbra at 1 d after injury in tissues obtained from sham-operated, vehicle control and FFA-treated mice. There was almost no obvious Trpm4 expression in the capillaries (labeled with CD31) of the sham-operated mice. As shown in the vehicle control and FFA-treated mice, Trpm4 was prominently up-regulated in the capillaries. Scale bar = 50 μm (left images) and 15 μm (right, higher magnification images, as marked in a white box in the left images). (B) RT-PCR for Trpm4 mRNA at 1 d after injury. β-actin was used as the control. (C) Bar graph showing the quantitative analysis of the RT-PCR results. (D) Western blots performed for the Trpm4 protein in tissues obtained at 1 d after SCI. β-actin was used as the control. (E) Bar graph showing a densitometry analysis of the results of the Western blotting experiments. The error bars represent the SD. *p<0.05, **p<0.01, and ***p<0.001 by one-way ANOVA followed by Tukey's post hoc analysis.