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. 2018 Jul 16;8(15):4050–4061. doi: 10.7150/thno.21524

Figure 5.

Figure 5

Wild-type IDH2 promotes the Warburg effect and cell growth through HIF1α. (A) Ubiquitinated and total HIF1α protein levels were determined by Western blotting in H460 and A549 lung cancer cells overexpressing IDH or expressing knockdown IDH2. β-Actin served as a loading control. (B) HIF1α protein levels were determined by Western blotting in H460 lung cancer cells overexpressing IDH2 and treated with DMSO or 1 mM octyl-α-KG. β-Actin served as a loading control. (C) Intracellular α-KG levels were determined in H460 lung cancer cells overexpressing IDH2 and treated with DMSO or 1 mM octyl-α-KG (** p < 0.01). (D) Glucose uptake and lactate production were determined in H460 and A549 lung cancer cells in the presence of the HIF1α inhibitor, PX-478 (10 μM) (** p < 0.01).(E) Cell number was determined by Trypan blue staining in H460 and A549 lung cancer cells overexpressing IDH2 in the presence of PX-478 (10 μM) (** p < 0.01).