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. 2018 Jul 16;8(15):4050–4061. doi: 10.7150/thno.21524

Figure 6.

Figure 6

Targeting wild-type IDH2 and replenishing cellular α-KG decreases cell viability and growth. (A) The growth inhibition rates of different human lung cancer cells and normal MRC5 lung fibroblasts in the presence of AGI-6780. (B) IDH activity was analyzed in H460 and A549 lung cancer cells treated with DMSO or AGI-6780 (20 μM) for 24 h (** p < 0.01). (C) Cell viability and cell number of H460 and A549 lung cancer cells treated with different concentrations of AGI-6780 (0-50 μM). (D) Cell number was determined by Trypan blue staining in H460 and A549 lung cancer cells in the presence of 5 μM AGI-6780 (** p < 0.01). (E-F) Glucose uptake, lactate production and cell number were determined in H460 and A549 lung cancer cells treated with 1 mM octyl-α-KG (** p < 0.01). (G) Cell number was determined by Trypan blue staining in H460 and A549 cells transfected with siRNA targeting HIF1α (* p < 0.05, ** p < 0.01).