Table 2.
Overview of RT-qPCR primer sequences and microarray probe sequences used to quantify lncRNAs in liquid biopsies.
| gene | reference | forward primer | reverse primer | LNCipedia ID | remark |
|---|---|---|---|---|---|
|
MALAT1 (MD-miniRNA) |
Ren et al., 2013 | CTTCCCTAGGGGATTTCAGG | GCCCACAGGAACAAGTCCTA | MALAT1 | lnc-LTBP3-2 on other strand |
|
MALAT1 (MD-miniRNA) |
Xue et al., 2015 | not mentioned | not mentioned | MALAT1 | lnc-LTBP3-2 on other strand |
| MALAT1 | Wang et al., 2014 | CTTCCCTAGGGGATTTCAGG | GCCCACAGGAACAAGTCCTA | MALAT1 | lnc-LTBP3-2 on other strand |
| FR0348383 | Zhang et al., 2015 | TAAACCTCCTTATCACATGCAGAA | GGACACCGTAGATTCTAGGACACT | not available | |
| SCHLAP1 | Prensner et al., 2014 | TGGACACAATTTCAAGTCCTCA | CATGGTGAAAGTGCCTTATACA | SCHLAP1 | |
| GAS5 | Isin et al., 2015 | CTTCTGGGCTCAAGTGATCCT | TTGTGCCATGAGACTCCATCAG | GAS5 | |
| lincRNA-p21 | Isin et al., 2015 | GGGTGGCTCACTCTTCTGGC | TGGCCTTGCCCGGGCTTGTC | not available | |
| PCAT18 | Crea et al., 2014 | AGGAGACAGGCCCCAGATTT | TGAAGTGCTGGGACAACGTA | PCAT18 | AQP4-AS1 on other strand |
| AK024556, XLOC_007697 | Lee et al., 2014 | ENSG00000274721 TTGGAGCCATTGTACGTGTCGGGAACATATCAGAACACCGAGAATAGCGTCATGTCATAA |
(chr5:141697230–141697290) (chr9:044182789–044182730) |
lnc-FGF1-9 lnc-SPATA31A6-6 |
A_21_P0006269 |
| LOC100287482 | ACTTCCTCTGTTATGCCAGATATGGTTAGCCACTTTGGTTTTTTAGGAGCTATAGGATGG | (chr7:129152443–129152502) | protein coding (SMKR1) | A_21_P0013271 (NM_001195243) | |
| XLOC_005327 | AGAGCTCAAAAGGCAAGAAATCAGCAAGAGAGAGAGATGAAGCATGAGAAATGAGCAAAA | (chr6:53495838–53495897) | LINC01564 | A_19_P00802433 | |
| XLOC_008559 | CATGTGTGTGCATGTGAGAGAGAGAGAGAAATAGGTTTTAATCCTTTGTTCTTTTCTTAT | (chr10:92749981–92750040) | lnc-RPP30-2 | A_21_P0007070 | |
| XLOC_009911 | AAGTGAATCTGGAGCTTAGATGGAGAGAGAAGAGAGAGATTAATTGAGGCCCCAGGTACT | (chr12:121343059–121343118) | lnc-HNF1A-1 | A_21_P0007854 |