Fig. 1.

Deep sequencing analysis of saliva RNA for Y4-RNA fragments. (A) Human full-length Y4-RNA and its fragments. Arrowheads on the 94-nt Y4-RNA denote the cleavage sites to generate 31-, 14-, and 11-nt fragments, which can function as 5′-half-tRNA-type, linear-type, and linear-type sgRNAs, respectively, for tRNase Z^L. The expected cleavage sites by tRNase Z^L are indicated by thin arrows in model target RNAs. (B) Frequencies of Y4-RNA fragments in saliva from four healthy persons, E1–E4. Read numbers of the 9–34-nt fragments are presented.