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. 2017 Jul 26;2(2):122–128. doi: 10.1016/j.ncrna.2017.07.002

Fig. 2.

Fig. 2

Quantitative RT-PCR. (A) Primer pairs. The sequences of the primers SL-forward_Y4_3 and Y4RNA_R4 for amplification of the 31/32-nt Y4-RNA fragment and the sequences of the primers SL-forward_Y4_3 and Y4RNA_R5 for amplification of the full-length Y4-RNA are presented together with the sequence of Y4-RNA, a middle part of which is omitted. Three nucleotides are added to the 5′ terminus of each primer to stabilize the primer/template hybrid. (B) A representative RT-PCR data for the synthetic 31-nt Y4-RNA fragment (0.001, 0.01, 0.05, and 0.1 fmol) and saliva RNA (1 ng) from the examinees E1, E3, and E4. The right and left halves represent the data for the full-length Y4-RNA and the 31/32-nt fragment, respectively. (C) A standard curve was drawn using the RT-PCR data for the synthetic 31-nt Y4-RNA fragment. (D) Quantitation of the 31/32-nt Y4-RNA fragment in saliva RNA from the examinees E1, E3, and E4. Error bars denote standard deviations (n = 3).