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. 2017 Jul 26;2(2):122–128. doi: 10.1016/j.ncrna.2017.07.002

Fig. 4.

Fig. 4

Fluorometric quantitation with molecular beacons. (A) DNA molecular beacons for human Y4-RNA fragments. The hybrid of the 31-nt Y4-RNA fragment and Beacon-1 or Beacon-2 is shown. F, 6-carboxyfluorescein; G, dabcyl. (B) Reaction time dependency of molecular beacon fluorescence. Beacon-2 (10 pmol) was incubated with Target-2 (4 pmol) or the synthetic 31-nt Y4-RNA fragment (4 pmol) at room temperature. (C) Sensitivity tests. Ten pmol of Beacon-1 (left bars) or Beacon-2 (right bars) were incubated at room temperature for 25 min with 4 pmol of the synthetic 31-nt Y4-RNA fragment without or with 2′-O-methyl modifications, or Target-1–Target-5. (D) Non-specific target effects. One (left bars), 0.4 (middle bars), or 0.1 (right bars) pmol of Beacon-2 were incubated at room temperature for 25 min with the synthetic 31-nt Y4-RNA fragment (4 pmol) and/or a Target-3–Target-5 mixture (1, 2, or 3 pmol each).