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. 2018 Aug 1;74(Pt 8):506–511. doi: 10.1107/S2053230X18008841

Table 2. Crystallization of CmpR-EBD.

Method Hanging-drop vapour diffusion
Plate type 96-well plate
Temperature (K) 289
Protein concentration (mg ml−1) 11
Buffer composition of protein solution 20 mM Tris–HCl pH 8.0, 200 mM NaCl
Composition of reservoir solution 0.1 M Tris pH 8.5, 25% t-butanol
Volume and ratio of drop 1 µl:1 µl
Volume of reservoir (µl) 500