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. 2018 Jul 26;74(Pt 8):480–489. doi: 10.1107/S2053230X1800537X

Table 2. Crystallization.

Method Vapour diffusion, sitting drop
Plate type Cryschem 24-well plate
Temperature (K) 293
Protein concentration (mg ml−1) 5
Buffer composition of protein solution 0.5 mM CQH, 5%(v/v) DMSO, 250 mM sodium acetate pH 5.5
Composition of reservoir solution 20%(w/v) PEG MME 5000, 0.2 M sodium sulfate
Volume and ratio of drop 1 µl HLE–CQH solution:0.5 µl reservoir solution
Volume of reservoir (µl) 200