Table 2. Crystallization.

	RtGH124–G3–Mn (PDB entry 6g1g)	RtGH124–G5F–Mn (PDB entry 6g1i)
Method	Vapour diffusion, sitting drop	Vapour diffusion, sitting drop
Plate type	96-well 2-drop MRC	96-well 2-drop MRC
Temperature (K)	293	293
Protein concentration (mg ml−1)	40	40
Volume and ratio of drop	54 µl, 1:1 ratio	54 µl , 1:1 ratio
Volume of reservoir (nl)	400	400
Buffer composition of protein solution	20 mM Tris pH 7.5, 6.7 mM G3	20 mM Tris pH 7.5, 6.7 mM G5F
Composition of reservoir solution	12% Tacsimate pH 5.0, 18% PEG 3350, 2 mM manganese(II) acetate	6% Tacsimate pH 5.0, 20% PEG 3350, 2 mM manganese(II) acetate
Cryoprotection buffer solution	14% Tacsimate pH 5.0, 19% PEG 3350, 2 mM manganese(II) acetate	8% Tacsimate pH 5.0, 21% PEG 3350, 2 mM manganese(II) acetate