Figure 3.

Examples of regions where bulk remodelling was necessary. (a) Presumably owing to an erroneous sequence alignment to the homology template, Lys468 of chain 4 was missing, with the following 27 residues shifted in register by one residue to fill the gap. This was corrected using ISOLDE’s register-shift function prior to adding the missing residue in Coot. (b) Final conformation after refinement against the autosharpened map. (c) The zinc-finger domain in chain 6 is found in weak and fragmented density, and was originally modelled as a polyalanine trace with the cysteine residues out of position. (d) Autosharpening of the map significantly improved interpretability in this region, allowing hand-modelling of this domain into the canonical fold with the coordinated zinc ion present in the centre of the highest density peak.