Fig. 3.

2D Aliphatic ¹³CH HSQC spectra of MSGs selectively labeled with a [β-¹³C; β₂-D]-Trp or b [β-¹³C; β₃-D]-Trp, and otherwise fully deuterated. Sample: 0.12 mM in 20 mM sodium phosphate buffer (pH 7.1), containing 20 mM MgCl₂, 5 mM DTT, and 5% D₂O. NMR: d1 = 4 s, TD = 1024 × 128, 310 K