Fig. 5.

Effect of the implementation of the additional NOE constraints acquired for the aromatic and aliphatic methylene protons on the NMR structure of MSG. Structural calculations including NOE constraints for a ILV methyls and backbone amides; b ILV methyls, backbone amides, and aromatic ring protons of Phe, Tyr, and Trp residues. Only the relative orientation between F35 and W36 is illustrated, although the overall structure was substantially refined by including the NOEs involving aromatic and aliphatic protons (details will be published elsewhere)