Fig. 6.

900 MHz 1D ¹H-NMR spectrum of [U-D;δ,ε-D₄-Phe]-MSG. Sample: 0.2 mM in 20 mM deuterated sodium phosphate buffer (pH ~ 7), containing 20 mM MgCl₂ and 5 mM DTT. NMR: d1 = 30 s, NS = 512, 310 K. The total experimental time was ~ 4.5 h. The signal assignments were transferred directly from the 2D aromatic ¹³CH TROSY spectrum shown in Fig. 2