Table 2.
Signalling pathways affected by PFOS treatment. KEGG pathway information generated from analysis in STRING using genes whose expression showed ≥ two-fold regulation (p ≤ 0.05) at either of the two administered doses of PFOS, including the proportion of genes analysed within these pathways that met the aforementioned criteria. Fraction of affected genes refers to the number of differentially affected genes compared to the total number of genes in that pathway that were analysed in the array (refer Table S2). The false discovery rate (calculated by STRING) is an indication of the likely proportion of false positive gene matches for the specified pathway
| KEGG ID | Pathway description | Observed genes | Fraction of affected genes | False discovery rate |
|---|---|---|---|---|
| 650 | Butanoate metabolism | ACAT1, ACAT2, ACSM3, ACSM5, HMGCS2, HADHA, HMGCL | 7 of 13 | 6.43E-14 |
| 3320 | PPAR signalling pathway | ACSBG1, ACSL6, CPT2, FABP3, FABP4, FABP5, FABP7, SLC27A1 | 8 of 28 | 2.01E-13 |
| 71 | Fatty acid degradation | ACAA2, ACAT1, ACAT2, ACSBG1, ACSL6, CPT2, HADHA | 7 of 22 | 3.28E-13 |
| 280 | Valine, leucine and isoleucine degradation | ACAA2, ACAD8, ACAT1, ACAT2, HMGCS2, HADHA, HMGCL | 7 of 14 | 1.73E-12 |
| 1212 | Fatty acid metabolism | ACAA2, ACAT1, ACAT2, ACSBG1, ACSL6, CPT2, HADHA | 7 of 27 | 2.00E-12 |
| 72 | Synthesis and degradation of ketone bodies | ACAT1, ACAT2, HMGCS2, HMGCL | 4 of 7 | 7.63E-09 |
| 1100 | Metabolic pathways | ACAA2, ACAD8, ACAT1, ACAT2, ACOT8, ACSBG1, ACSL6, ACSM3, ACSM5, HMGCS2, HADHA, HMGCL | 12 of 40 | 9.17E-09 |
| 900 | Terpenoid backbone biosynthesis | ACAT1, ACAT2, HMGCS2 | 3 of 4 | 2.22E-05 |
| 640 | Propanoate metabolism | ACAT1, ACAT2, HADHA | 3 of 8 | 0.000115 |
| 380 | Tryptophan metabolism | ACAT1, ACAT2, HADHA | 3 of 5 | 0.000188 |
| 1120 | Microbial metabolism in diverse environments | ACAA2, ACAT1, ACAT2, HADHA | 4 of 10 | 0.000188 |
| 310 | Lysine degradation | ACAT1, ACAT2, HADHA | 3 of 5 | 0.000237 |
| 4146 | Peroxisome | ACOT8, ACSL6, HMGCL | 3 of 16 | 0.00107 |
| 1200 | Carbon metabolism | ACAT1, ACAT2, HADHA | 3 of 8 | 0.0017 |
| 62 | Fatty acid elongation | ACAA2, HADHA | 2 of 4 | 0.00271 |
| 630 | Glyoxylate and dicarboxylate metabolism | ACAT1, ACAT2 | 2 of 4 | 0.00456 |
| 620 | Pyruvate metabolism | ACAT1, ACAT2 | 2 of 6 | 0.00917 |
| 4920 | Adipocytokine signalling pathway | ACSBG1, ACSL6 | 2 of 13 | 0.0213 |