Fig. 4.
Reporter protein activity after stimulation of BV-2 cells transduced with the polarisation reflecting promoters. a, b M1-like iNOS and Fcgr3 promoters were activated by LPS + IFN-γ with a significant increase after 4 h and 12 h, and after 4 h, respectively. c M2-like Ym1 promoter was significantly activated by IL-4 stimulation, but down-regulated by LPS + IFN-γ at 12 h and 24 h. Luc2 and eGFP levels were measured using a Mithras2 LB943 plate reader (n = 6 wells; 3 independent experiments for each group). Signal intensity values of stimulated conditions were expressed in relative units to PBS condition, set to 100%. Mean ± standard deviation is shown. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001