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Y2H Assay of the Interaction between Hrip1 (A. alternata R2) and MdNLR16.
The pGADT7-T and pGBKT7-p53 pair was used as a positive control. Pairs of AD-Hrip1 and BD-MdNLR140, and AD-pGADT7 and BD- pGBKT7 were used as negative controls. Each colony was dissolved in 10 μL sterile water and then diluted to 10−1 to 10−3. At least three colonies per combination were tested.
