. 2018 May 31;131(9):1925–1938. doi: 10.1007/s00122-018-3123-5

Table 1.

Primer sequences and restriction conditions to detect five molecular markers associated with Ry genes

Marker	Ry gene	Primer name	Sequence (5′–3′)	T°a	Digestion	Amplicon size (bp)	References
RYSC3	Ry _adg	3.3.3 S	ATACACTCATCTAAATTTGATGG	60	_	321	Kasai et al. (2001)
RYSC3	Ry _adg	ADG23R	AGGATATACGGCATCATTTTTCCGA	60	_	321	Kasai et al. (2001)
M5	Ry _sto	M5-m	GCTGTTCACAATGGGAACATGG	60	MseI/BSA	485	Brigneti unpublished
M5	Ry _sto	M5-p	CATACAAACTACTTCTACCACG	60	MseI/BSA	485	Brigneti unpublished
M6	Ry _sto	M6 Rsa	TCCGAAATGTTTGGGCTGACATC	55	RsaI	1126	Brigneti unpublished
M6	Ry _sto	M6 Taq	AAGGGATCCAAAAAGGTGGTTCA	55	RsaI	1126	Brigneti unpublished
M17	Ry _sto	M17-m	GACTGCTTTCTCTCCACGTGGC	60	PstI	250	Brigneti unpublished
M17	Ry _sto	M17-l	GATCACAGATGTTTTACCTTCGATG	60	PstI	250	Brigneti unpublished
M45	Ry _sto	M45-m	CCTAGTTTCTGAGCATGTAATTTC	61	_	495	Brigneti unpublished
M45	Ry _sto	M45-p	TGCAGCTATTCAAAACACATAAGG	61	_	495	Brigneti unpublished
M6	Ry _adg	M6F1	ACATGATATAAGTTGATATGGAGAAT	60	_	994	This article
M6	Ry _adg	M6R4	GTGCTTTGTCTTTTCTGCATGTA	60	_	994	This article
M45	Ry _adg	M45F1	TGGAGTATTTGGATCTAAGGG	61	_	268	This article
M45	Ry _adg	M45R1	AACACATAAGGAGCGATG	61	_	268	This article
M6	Ry _adg	P2F1	TAGATACGCCACTCCACATA	60.5	_	135	This article
		P2R1	GAACCCATCCGTGATAAAT
		P2	GCTGCTCGGGGTCACCAC