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. 2018 Jul 17;67(9):789–799. doi: 10.1007/s00011-018-1171-6

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — Leptin-induced (a) and TNF-induced (b) mast cell migration. Mast cells were incubated with different concentrations of leptin, TNF (positive control) or medium alone (control spontaneous mast cell migration) for 3 h at 37 °C in a Boyden microchamber. Ten HPF were counted in each assay (×250). Spontaneous migration served as a control and was referred to as 100%. Each point represents the mean ± SD of three independent experiments done in duplicate. *P < 0.05