Table 1.

Checkerboard analysis of leptin-induced mast cell migratory response

Leptin in lower compartment (ng/ml)	Leptin in upper compartment (ng/ml)
	0	0.01	0.1	1
0	100 ± 25	99 ± 10	104 ± 11	86 ± 5
0.01	146 ± 9*	95 ± 10	94 ± 4	91 ± 6
0.1	318 ± 22*	108 ± 6	113 ± 6	91 ± 7
1	127 ± 9	109 ± 8	101 ± 8	90 ± 8

Mast cells were incubated with different concentrations of leptin or medium alone (control spontaneous mast cell migration) for 3 h at 37 °C in a Boyden microchamber. Ten HPF were counted in each assay (×250). Spontaneous migration served as a control and was referred to as 100%

Each point represents the mean ± SD of three independent experiments done in duplicate (n = 6)

*P < 0.05