Fig. 3.

Mg²⁺ deficiency prevents diet-induced hepatic lipid storage. Liver (a) weight and (b) triacylglycerol content at death (17 weeks, n = 12 mice per group, n = 11 mice for the LowMg-HFD group). Representative images of livers stained with (c) H&E or (d) Oil Red O. Scale bars, 100 μm. NormMg-LFD (white circles), LowMg-LFD (grey circles), NormMg-HFD (white squares), LowMg-HFD (grey squares). Data are mean ± SEM. Depending on the absence or presence of a significant interaction effect between dietary fat and Mg²⁺ content, either a two-way ANOVA (Tukey’s multiple comparison test) or an unpaired multiple t test (Holm–Sidak multiple comparison test) approach was used to determine statistical significance, respectively. *p < 0.05 for the comparisons shown