Fig. 6.

Quantification of the total area of insulin, glucagon, somatostatin and pancreatic polypeptide (PP) immunoreactivity within native islet (a) and CFA-PI (b) grafts recovered 60 days post-transplant (DAPI⁺ within the graft area, each symbol representing one transplant). Interestingly, despite an equivalent or increased capacity to restore normoglycaemia, CFA-PI grafts showed an increased total PP-positive area and a decreased total insulin-positive area (both *p < 0.05) compared with that of native islet grafts. (c) Fluorescent images of human native islet grafts and human CFA-PI grafts stained positive for insulin (red), glucagon (green), somatostatin (magenta), PP (cyan) and nuclei (DAPI, grey) (scale bar, 50 μm). Data from animals transplanted with n = 6 independent human islet/CFA-PI preparations. Differences were compared by unpaired t test