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. 2018 Aug 1;410(23):5825–5837. doi: 10.1007/s00216-018-1199-z

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Influence of spatial resolution and imaging accuracy in a measurement of mouse brain cerebellum with 25 μm pixel size shown on m/z = 536.316703 (tryptic peptide of tubulin polymerization-promoting protein). a–c selected ion images recalculated for different acquisition parameters. d Selected ion image measured with 25 μm pixel size and generated with a small bin width of Δm/z = ± 0.005 U. e ALLEN mouse brain atlas: in situ hybridization of tubulin polymerization-promoting protein. f H&E staining of an adjacent section