Table 1.
Media composition [1Driver and Kuniyuki (1984); 2McCown and Lloyd (1981); 3Murashige and Skoog (1962)]
| Induction | Multiplication | Expression | Maturation | Germination | ||
|---|---|---|---|---|---|---|
| Code | PCG | SCG | CC21 | CC2 | G80 | ENR8 |
| Macronutrients | DKW(1) | WPM(2) | MS(3) | MS(3) | MS(3)/2 | MS(3) |
| Micronutrients | DKW(1) | WPM(2) | DKW(1) | DKW(1) | MS(3) | DKW(1) |
| Vitamins (mg L−1) | ||||||
| Myo-inositol | 200.0 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 |
| Nicotinic acid | 1.0 | 1.0 | 1.0 | 1.0 | 0.5 | 1.0 |
| Thiamine | 2.0 | 10.0 | 2.0 | 2.0 | 0.7 | 2.0 |
| Pyridoxine-HCl | 1.0 | 0.5 | ||||
| Amino acids (mg L−1) | ||||||
| Glycine | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 |
| L-Lysine | 0.4 | 0.4 | 0.4 | 0.4 | ||
| L-Leucine | 0.4 | 0.4 | 0.4 | 0.4 | ||
| L-Arginine | 0.4 | 0.4 | 0.4 | 0.4 | ||
| L-Tryptophane | 0.2 | 0.2 | 0.2 | 0.2 | ||
| Glutamine | 250.0 | |||||
| Plant growth regulators (mg L−1) | ||||||
| 2,4,5-T | 1.000 | |||||
| 2,4-D | 2.000 | 2.000 | ||||
| Naphthaleneacetic acid | 0.010 | |||||
| Kinetin | 0.250 | |||||
| Thidiazuron | 0.005 | |||||
| Isopentenyl adenine | 50.000 | 0.200 | ||||
| Adenine-H2SO4 | 0.250 | 0.025 | ||||
| Abscisic acid | 1.000 | |||||
| Gibberellic acid | 0.020 | |||||
| Coconut milk (mL L−1) | 50.0 | |||||
| Other (g L−1) | ||||||
| Activated charcoal | 1 | |||||
| Glucose | 20 | 20 | 30 | 30 | 40 | 30 |
| Gelrite® | 3 | 3 | 3 | 3 | ||
| pH | 5.6 | 5.6 | 5.6 | 5.6 | 5.8 | 5.8 |