Fig. 1.

Ontogeny of membrane potential and Kv7.2 and Kv7.3 channel protein expression in mouse striatal neurons isolated at embryonic stages E13, E15 and E17 and differentiated in vitro. a Exponential fits of membrane potential (Vm) changes in mouse striatal neurons isolated at embryonic stages E13 (black circles), E15 (grey triangles) and E17 (empty squares) and cultured for up to 15 days post-plate down (dpp). The dashed boxes represent the time windows for which the analyses in b have been derived. b Bar charts presenting means of Vm measured in mouse striatal neurons isolated at the embryonic stages indicated above each bar (E13 (black), E15 (grey) and E17 (white)) and assayed at 1 dpp (left), 7 dpp (centre) and 14 dpp (right). Number of observations shown in parentheses. Significant differences, determined by unpaired Student’s t test, are shown below the bars, where appropriate; ****p < 0.0001. c Kv7.2 (left panels, ×20 and ×100, as indicated) and Kv7.3 (right panels, ×20 and ×100, as indicated) protein expression by immunocytochemistry in E15 mouse striatal neurons during in vitro maturation at 1, 7 and 14 dpp