Fig. 3.

Ontogeny of KCNQ mRNA expression in developing mouse and human striatum. a KCNQ mRNA expression in mouse striatal neurons during development in vivo. mRNA levels were determined in mouse striatal neurons isolated at E13, E15 and E17 by qPCR and were normalised to the house-keeping genes (18S/β-actin) using the ΔΔCt method. By one-way ANOVA (Tukey’s post hoc), there were significant differences between KCNQ mRNA at E15 and E17 with its own control at E13, p < 0.05. b KCNQ mRNA expression in E13 mouse striatal neurons during in vitro differentiation and maturation. By one-way ANOVA (Tukey’s post hoc), there were significant differences of KCNQ2 and KCNQ3 mRNA between 1 and 7 dpp, p < 0.05. c KCNQ mRNA expression during human striatal development in vivo. mRNA levels in human striatal regions dissected from cryosections were determined by qPCR and were normalised to house-keeping genes (RPL13A and HSP90AB1) using the ΔΔCt method. By unpaired Student’s t test, there were significant differences between whole ganglionic eminence (WGE, 7–9 weeks post-conception) and adult striatum expression of KCNQ3, KCNQ4 and KCNQ5 mRNA, *p < 0.05